The product is mainly composed of mechanical control device, photon receiving and converting device, signal analysis and processing circuit, embedded software (release version NO. V1.2), display device and power module.
Sample feeding port: it is the only outlet between the product and the outside world to complete the sample bearing and light protection effect sample plate transmission: responsible for the sample to be tested to the detection unit optical fiber transmitter: optical fiber transmitter transmits the photon signal of a single sample to the photon counting component, effectively isolating the light pollution between samples Photon counting component: photon counting component to complete the collection and pretreatment of the photon signal emitted by the sample motion controller: used for the X and Y axis transmission of the sample to be tested to the test position; High voltage generator: provides 1200V DC voltage data for photon counting module; Processor: completes data amplification, shaping, detection, threshold control, level transformation, data buffering Computer unit: the computer unit is used for system control, and the special testing software is used to complete the mechanical action control data acquisition, data processing, fitting and calculation results display, printing, storage, etc
Chemiluminescence is the emission of light produced by chemical reactions at room temperature. Chemiluminescence is a multi-step process whereby certain compounds (luminescent agents or substrates) can use the energy generated by a chemical reaction to raise the product molecule or intermediate molecule of the reaction to an electron excited state. When the product molecule or intermediate molecule decays to the ground state, it releases energy in the form of emitted photons (i.e., light). There are two types of chemiluminescence systems. One uses enzymes to produce chemiluminescence signals. Another chemiluminescence system uses non-enzyme direct chemiluminescence labeling.
For the chemiluminescence signal generation system using enzymes, the commonly used luminescence reagents are aminophendiamide hydrazine (mainly luminol and isiluminol derivatives) and acridine ester. The success of chemiluminescence determination of isoluminol labeled biotin in 1976 led to the rapid development of chemiluminescence immunoassay and the rapid improvement of detection sensitivity. The luminescence system that has been widely used in the past is shown below:
Luminol generates amino phthalate ions in an excited state and releases photons when it returns to its ground state.
The luminescence system composed of the above three parts has been widely used in many fields for a long time, but due to its low sensitivity, it cannot be used to detect trace amounts of proteins and nucleic acids. It was not until the mid – to late 1980s that the technique was quickly applied to genetic analysis and immunoassay after the discovery of two classes of substances that greatly enhanced the intensity of their luminescence. One is 6-hydroxyl benzothiazole and its derivatives, the other is a para-substituted phenols, such as p-iodine-phenol, p-phenylphenol and so on. The enhancement effect of these two kinds of substances is very high, can increase the luminous intensity more than a thousand times, but also can greatly reduce the oxidizer and luminous agent alone when the “background” luminous, and can prolong the luminous time for several hours. Therefore, they are widely used in chemiluminescence technology. Typically, the system uses alkaline phosphatase or horseradish peroxidase, adding a substrate that emits visible light in response to the enzyme. The luminous flux is determined by the substrate. The process is as follows:
Light Emission Mechanism of CSPD
For the non-enzyme direct chemiluminescence labeling system, due to its small background signal, the signal can be excited in a shorter time. Chemiluminescence usually speeds up the determination by a factor of ten or more, or increases the sensitivity by a factor of more than ten.
2.2 Reaction Process
Quantitative human body fluids and enzyme markers were added to a white opaque enzyme label plate containing solid phase antibodies. The molecules and enzyme markers in body fluids were competitively bound to the solid phase antibodies. The unbound free components were separated and washed, and the luminescent substrate working solution was added, and the enzyme promoted the luminescence of the substrate. At this point, the enzyme plate is placed into the analyzer, and the luminescence value of each hole is read by the photon counting system in turn through the precise three-dimensional transmission mechanism inside the instrument. The molecular concentration to be measured in the sample was calculated quantitatively according to the mathematical model established by the standard substance, and finally the data report was printed out to assist clinical diagnosis.
Mains input： AC220V/50Hz Input power：300VA
Measuring range：In photon numbers,100RLU～50,000,000 RLU
Linear dependence：r＞0.99(At least 3 orders of magnitude)
Interference between hole：≤10-3；
Minimum response value：Reference light source luminous value≥2×background noise
Deviation between measured value and theoretical value of reference light source：≤10%
Measuring time： Less than 5 minutes (sampling in 1 second, 96 samples tested)