Nucleic Acid Extraction Kit(Manual)

Short Description:

Product Introduction:

The product has high efficiency and recovery, can effectively extract theDNA/RNAof virus, and the purified nucleic acid being extracted is suitable forreverse transcription,PCR,RT-PCRand other common downstream experiments.

Product characteristics:

•No toxicity:There is no need of expensive equipment, and the exposure to toxic reagents like phenol and chloroform can be completely avoided, it can be operated in the laboratory and outside.
• Wide range: It is suitable for DNA/RNAextraction of serum, plasma, urine and other cell-free body fluids and virus culture fluid, cell culture fluid, virus preservation fluid and other samples.
• Easy operation:The extraction process is easy to operate and automate, and suitable for high-throughput extraction.

Product Specifications:

Automatic

48 tests/box

Manual

50 tests/box 


  • Product name: Nucleic Acid Extraction Kit(Automatic)
  • Packing specification: 50 tests/box
  • Product Detail

    Product Tags

    【Intended use】
    In this kit, guanidine isothiocyanate is used for pyrolysis, total DNA and RNA in samples are separated and purified by magnetic beads adsorption, and PCR inhibitors such as proteins, lipids and ribonuclease can be effectively removed after purification. The purified products can be directly used for fluorescence quantitative PCR.

    【Inspecting principle】
    In this kit, guanidine isothiocyanate is used for lysis, nucleic acid is released, nucleic acid is absorbed by electrostatic effect, hydrophobic effect and hydrogen bond effect, and PCR inhibitors such as proteins, lipids and ribonuclease are removed after washing。

    【Extraction Method】
    1. Preparation of reagent board
    (1) Take out the prepackaged reagent board from the kit and turn it upside down several times to make the magnetic beads suspend evenly;
    (2) Carefully tear off the aluminum foil sealing film to avoid the vibration of orifice plate and prevent the liquid from splashing out.
    2. Sample preparation
    100 μ l of sample was added to deep well plate station 1, and 10 μ l of protease K and 2 μ l of carrier RNA were added to the cleavage tank.
    3. On machine extraction
    put the prepared 96 deep well plate in the nucleic acid extractor, insert the magnetic rod sleeve, and extract.





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