SARS-CoV-2 Constant Temperature PCR Detection kit

Short Description:

Product introduction:

It is used to test the sample to be tested forthe special loci of novel coronavirus nucleic acids (ORF1ab,Ngene).

Product characteristics:

•Easy :Easy to operate, learn, and understand, no complex training is needed.
•Isothermal :Save instrument cost.
•High Specificity :D etection accuracy as high as 98%.
•Rapid :The detection can be completed within 15 minutes.
•Convenient transport and storage :room temperature transportation and storage, no cold chain.

Product specifications:

1 test/box、16 tests/box

①swab②Swab preservation tube③Amplification reaction tube④metal bath


  • Product name: SARS-CoV-2 Constant Temperature PCR Detection kit(Home use)
  • Type: Constant Temperature PCR
  • Packing specification: 1 test/box、16 tests/box
  • Product Detail

    Product Tags

    Test principle:
    This kit detects the RNA of SARS-CoV-2 using an isothermal amplification method. The reverse transcription and mplification of RNA are performed in the same tube. The nucleic acid sequence of SARS-CoV-2 is specifically identified by six primers, and any primer mismatch or unpaired will not complete the amplification. All the reagents and enzymes required for the reaction are pre-loaded. Simple process is needed and the result can be derived by observation on the presence or not of fluorescence.

    Preparation:

    Open the Aluminum foil bag and take out the reaction tubes. Attention, the reaction tube must be used within 2 hours once its foil pouch is opened.

    Plug in the power. The instrument starts heating (The heating indicator turns red and flashes). After the heating process, the heating indicator turns green with a beep.

    Sample collection:

    Tilt the patient’s head back about 70°, Let the patient’s head relax naturally , and slowly rotate the swab against the wall of the ostril into the patient’s nostril to the nasal palate, and then slowly remove it while wiping.

    Test:
    ①Tear the aluminum foil seal film of Swab Preservation tube, and insert the swab into a swab preservation tube. while squeezing the tube, stir the swab.
    ②Remove the swab while squeezing the sides of the tube to extract the liquid from the swab.
    ③Squeeze the micropipette and put it into the liquid. Release the micropi-pette to draw liquid until the liquid flows into the first capsule . Don’t let the liquid fill the first capsule.
    ④Add the specimen liquid into the reaction tube, Close the cap, gently mix the mixture until completely dissolved .
    ⑤Open the cover of the dry bath. Put the capped reaction tubes into dry bath. Press the timing button. The green heating indicator starts to flash. After 15min, the reaction is completed. The green heating indicator stops flashing with three beeps.
    ⑥Press the switch button of the light source and observe the test results through the observation hole in front of the dry bath to judge the results.
    Interpretation of test results:

    Positive result: if the reaction tube has obvious green fluorescence excitation, the result is positive.The patient is suspected of being infected with Sars-Cov-2. Immediately contact doctor or local health department and follow local guidelines.
    Negative result: if the reaction tube doesn’t have obvious green fluorescence excitation, the result is negative.Continue to adhere to all applicable rules regarding contact with others and protective measures.There may also be the infection when tested negative.
    Invalid result: if the incubation time is longer than 20 min, non-specific amplification may occur, leading to a false positive.It will be invalid regardless of if there is obvious green fluorescence, and the test shall be conducted again.







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