In this kit, guanidine isothiocyanate is used for pyrolysis, total DNA and RNA in samples are separated and purified by magnetic beads adsorption, and PCR inhibitors such as proteins, lipids and ribonuclease can be effectively removed after purification. The purified products can be directly used for fluorescence quantitative PCR.
In this kit, guanidine isothiocyanate is used for lysis, nucleic acid is released, nucleic acid is absorbed by electrostatic effect, hydrophobic effect and hydrogen bond effect, and PCR inhibitors such as proteins, lipids and ribonuclease are removed after washing.
Storage conditions and period of validity
The kit was stored at 4℃, and the expiry date was 12 months.Heat preservation packaging shall be used for transportation, and pre-cooled low-temperature ice bags shall be added at -20℃, with the amount of ice bags not less than 20% of the total volume. Sealed container shall be transported for no more than 72 hours.
Product Performance Index
According to clinical assessment, the DNA detection sensitivity of this kit is 500IU/mL;The detection sensitivity of RNA was 500IU/mL.Hemolysis, hyperlipidemia, high bilirubin and high immunoglobulin samples should be avoided in this kit.
Remove the lysate, detergent, eluent and magnetic beads from the kit and place them at room temperature to dissolve them fully.
(2)RNA extraction steps
1.According to the number of specimens to be measured and the number of quantitative standards, a 1.5mL centrifuge tube was taken and labeled. Lysate 100 μL and magnetic bead 5 μL were added, respectively. Oscillating blending.
2.Add 50μ L specimen or standard, suspension and incubate at 60℃ for 10 minutes;
3.Place the cap of the centrifuge tube on the magnetic separator rack for 30 seconds and remove the supernatant;
4.Add 150 μL detergent, mix well, place the centrifuge tube cover on the magnetic separator rack for 30 seconds, and remove the supernatant;
5.Add 150 μL eluent, mix well, place the centrifuge tube cover on the magnetic separator rack for 30 seconds, and take the supernatant for reserve.
1.Before the experiment, the kit instructions should be read in detail.
2.In the process of specimen processing, the supernatant removal steps involved, please try to absorb the supernatant, while avoiding to absorb the precipitate, so as not to affect the accuracy of detection.
3.Samples should be considered as potential sources of infection, and the operation and treatment should meet the requirements of relevant regulations: General Guidelines for Biosafety of Microbial and Biomedical Laboratories and Regulations on the Management of Medical Wastes issued by the Ministry of Health.
【Index of CE Symbols】